TransMembrane prediction using Hidden Markov Models for Rv3764c




Residue number
and Amino Acid
Probabilities
InsideMembraneOutside
1 V0.841180.000000.15882
2 G0.841180.000000.15882
3 I0.841180.000000.15882
4 T0.841180.000000.15882
5 A0.841180.000000.15882
6 A0.841180.000000.15882
7 T0.841180.000000.15882
8 E0.841180.000000.15882
9 M0.841180.000000.15882
10 A0.841180.000020.1588
11 L0.841180.000020.1588
12 R0.841180.000020.1588
13 R0.841160.000040.1588
14 H0.841160.000040.1588
15 L0.841160.000130.15871
16 V0.841160.000130.15871
17 A0.841160.000130.15871
18 Q0.841160.000130.15871
19 L0.841160.000130.15871
20 D0.841160.000130.15871
21 N0.841160.000130.15871
22 Q0.841160.000130.15871
23 L0.841160.000130.15871
24 G0.841160.000130.15871
25 G0.841160.000130.15871
26 T0.841160.000130.15871
27 S0.841160.000130.15871
28 Y0.841160.000130.15871
29 R0.841160.000130.15871
30 S0.841160.000130.15871
31 V0.841160.000130.15871
32 L0.841170.000120.15872
33 M0.841240.000040.15872
34 Y0.841250.000020.15872
35 P0.841270.000010.15873
36 E0.841270.000000.15873
37 K0.841270.000000.15873
38 M0.841270.000000.15873
39 P0.841270.000000.15873
40 R0.841270.000000.15873
41 P0.841270.000000.15873
42 P0.841270.000000.15873
43 W0.841270.000000.15873
44 R0.841270.000000.15873
45 H0.841270.000000.15873
46 E0.841270.000000.15873
47 T0.841270.000000.15873
48 H0.841270.000000.15873
49 N0.841270.000000.15873
50 Y0.841270.000000.15873
51 I0.841270.000000.15873
52 R0.841270.000000.15873
53 S0.841270.000000.15873
54 G0.841270.000000.15873
55 P0.841270.000000.15873
56 G0.841270.000010.15873
57 P0.841270.000010.15873
58 R0.841260.000020.15872
59 F0.841250.000050.1587
60 L0.841250.000070.15869
61 D0.841240.000100.15866
62 A0.841220.001360.15743
63 P0.841200.004140.15466
64 G0.841150.007170.15167
65 Q0.841120.013300.14557
66 P0.840710.024120.13517
67 A0.839890.052350.10776
68 G0.839150.065130.09572
69 M0.839020.069910.09107
70 V0.838840.072100.08905
71 A0.838790.072610.0886
72 A0.838740.074510.08674
73 V0.838720.075070.08622
74 V0.838720.075120.08616
75 S0.838720.075150.08612
76 D0.838720.075190.08609
77 G0.838710.075540.08574
78 T0.838710.075700.08559
79 T0.838710.075730.08556
80 V0.838700.075750.08554
81 A0.838710.075750.08555
82 A0.838760.075680.08556
83 G0.838830.075600.08556
84 Y0.839110.075320.08558
85 L0.841280.073050.08566
86 T0.850270.063750.08597
87 G0.856430.057170.0864
88 S0.867150.046010.08683
89 G0.876450.036300.08725
90 S0.899360.012960.08767
91 R0.906660.005410.08793
92 A0.908020.004040.08795
93 A0.908700.003300.088
94 L0.908900.003080.08803
95 T0.911100.000830.08807
96 S0.911780.000120.08811
97 T0.911810.000080.08811
98 G0.911830.000060.08811
99 R0.911850.000040.08811
100 S0.911860.000030.08811
101 Q0.911880.000010.08811
102 L0.911880.000010.08812
103 E0.911890.000000.08812
104 R0.911890.000000.08812
105 I0.911880.000000.08812
106 A0.911880.000010.08812
107 G0.911880.000010.08812
108 S0.911880.000010.08812
109 R0.911880.000010.08812
110 T0.911880.000010.08812
111 P0.911880.000010.08812
112 L0.911880.000010.08812
113 T0.911880.000010.08812
114 L0.911880.000010.08812
115 D0.911880.000010.08812
116 L0.911880.000010.08812
117 D0.911880.000010.08812
118 G0.911880.000010.08812
119 L0.911880.000020.0881
120 G0.911880.000020.0881
121 R0.911880.000020.0881
122 Y0.911870.000030.0881
123 R0.911870.000030.08809
124 V0.911870.000050.08808
125 L0.911870.000050.08808
126 A0.911860.000060.08807
127 A0.911860.000070.08807
128 P0.911860.000070.08807
129 S0.911860.000080.08806
130 R0.911850.000090.08806
131 N0.911810.000130.08805
132 G0.911800.000150.08805
133 H0.911790.000150.08805
134 D0.911790.000160.08805
135 V0.911790.000160.08805
136 I0.911790.000160.08805
137 V0.911790.000170.08805
138 T0.911790.000160.08805
139 G0.911780.000160.08805
140 L0.911770.000180.08805
141 S0.911770.000180.08805
142 M0.911780.000170.08805
143 G0.911760.000190.08805
144 N0.911750.000200.08805
145 V0.911720.000290.08799
146 D0.911730.000310.08796
147 A0.911210.001210.08759
148 T0.910670.002150.08719
149 M0.909300.004100.08659
150 L0.901190.013400.08541
151 Q0.894850.021170.08398
152 M0.553010.400800.04618
153 L0.030890.964000.00511
154 I0.009070.988890.00203
155 I0.002910.995900.00119
156 F0.000620.998770.0006
157 G0.000260.999380.00036
158 I0.000010.999890.0001
167 A0.000050.999820.00012
168 T0.000260.999490.00025
169 T0.000360.999260.00038
170 A0.000470.998330.0012
171 G0.000580.996950.00246
172 I0.000690.994730.00459
173 V0.000910.984880.01421
174 I0.002250.966840.03091
175 I0.040660.584750.37459
176 K0.086080.008440.90548
177 R0.087530.001180.91128
178 A0.087620.000830.91155
179 L0.087680.000620.91169
180 A0.087860.000350.91179
181 P0.088050.000050.91189
182 L0.088070.000010.91193
183 R0.088070.000000.91193
184 R0.088070.000000.91193
185 V0.088070.000000.91193
186 A0.088070.000000.91193
187 Q0.088070.000000.91193
188 T0.088070.000000.91193
189 A0.088070.000000.91193
190 S0.088070.000000.91193
191 E0.088070.000000.91193
192 V0.088070.000000.91193
193 V0.088070.000000.91193
194 D0.088070.000000.91193
195 L0.088070.000000.91193
196 P0.088070.000000.91193
197 L0.088070.000000.91193
198 D0.088070.000000.91193
199 R0.088070.000000.91193
200 G0.088070.000000.91193
201 E0.088070.000000.91193
202 V0.088070.000000.91193
203 K0.088070.000000.91193
204 L0.088070.000000.91193
205 P0.088070.000000.91193
206 V0.088070.000000.91193
207 R0.088070.000000.91193
208 V0.088070.000000.91193
209 P0.088070.000000.91193
210 E0.088070.000000.91193
211 P0.088070.000000.91193
212 D0.088070.000000.91193
213 A0.088070.000000.91193
214 N0.088070.000000.91193
215 P0.088070.000000.91193
216 S0.088070.000000.91193
217 T0.088070.000000.91193
218 E0.088070.000000.91193
219 V0.088070.000000.91193
220 G0.088070.000000.91193
221 Q0.088070.000000.91193
222 L0.088070.000000.91193
223 G0.088070.000000.91193
224 S0.088070.000000.91193
225 A0.088070.000000.91193
226 L0.088070.000000.91193
227 N0.088070.000000.91193
228 R0.088070.000000.91193
229 M0.088070.000000.91193
230 L0.088070.000000.91193
231 D0.088070.000000.91193
232 H0.088070.000000.91193
233 I0.088070.000000.91193
234 A0.088070.000000.91193
235 A0.088070.000000.91193
236 A0.088070.000000.91193
237 L0.088070.000000.91193
238 S0.088070.000000.91193
239 A0.088070.000000.91193
240 R0.088070.000000.91193
241 Q0.088070.000000.91193
242 A0.088070.000000.91193
243 S0.088070.000000.91193
244 E0.088070.000000.91193
245 T0.088070.000000.91193
246 C0.088070.000000.91193
247 V0.088070.000000.91193
248 R0.088070.000000.91193
249 Q0.088070.000000.91193
250 F0.088070.000000.91193
251 V0.088070.000000.91193
252 A0.088070.000000.91193
253 D0.088070.000000.91193
254 A0.088070.000000.91193
255 S0.088070.000000.91193
256 H0.088070.000000.91193
257 E0.088070.000000.91193
258 L0.088070.000000.91193
259 R0.088070.000000.91193
260 T0.088070.000000.91193
261 P0.088070.000000.91193
262 L0.088070.000000.91193
263 A0.088070.000000.91193
264 A0.088070.000000.91193
265 I0.088070.000000.91193
266 R0.088070.000000.91193
267 G0.088070.000000.91193
268 Y0.088070.000000.91193
269 T0.088070.000000.91193
270 E0.088070.000000.91193
271 L0.088070.000000.91193
272 T0.088070.000000.91193
273 Q0.088070.000000.91193
274 R0.088070.000000.91193
275 I0.088070.000000.91193
276 G0.088070.000000.91193
277 D0.088070.000000.91193
278 D0.088070.000000.91193
279 P0.088070.000000.91193
280 E0.088070.000000.91193
281 A0.088070.000000.91193
282 V0.088070.000000.91193
283 A0.088070.000000.91193
284 H0.088070.000000.91193
285 A0.088070.000000.91193
286 M0.088070.000000.91193
287 S0.088070.000000.91193
288 R0.088070.000000.91193
289 V0.088070.000000.91193
290 A0.088070.000000.91193
291 S0.088070.000000.91193
292 E0.088070.000000.91193
293 T0.088070.000000.91193
294 E0.088070.000000.91193
295 R0.088070.000000.91193
296 I0.088070.000000.91193
297 T0.088070.000000.91193
298 R0.088070.000000.91193
299 L0.088070.000000.91193
300 V0.088070.000000.91193
301 E0.088070.000000.91193
302 D0.088070.000000.91193
303 L0.088070.000000.91193
304 L0.088070.000000.91193
305 L0.088070.000000.91193
306 L0.088070.000000.91193
307 A0.088070.000000.91193
308 R0.088070.000000.91193
309 L0.088070.000000.91193
310 D0.088070.000000.91193
311 S0.088070.000000.91193
312 G0.088070.000000.91193
313 R0.088070.000000.91193
314 P0.088070.000000.91193
315 L0.088070.000000.91193
316 E0.088070.000000.91193
317 R0.088070.000000.91193
318 G0.088070.000000.91193
319 P0.088070.000000.91193
320 V0.088070.000000.91193
321 D0.088070.000000.91193
322 M0.088070.000000.91193
323 S0.088070.000000.91193
324 R0.088070.000000.91193
325 L0.088070.000000.91193
326 A0.088070.000000.91193
327 V0.088070.000000.91193
328 D0.088070.000000.91193
329 A0.088070.000000.91193
330 V0.088070.000000.91193
331 S0.088070.000000.91193
332 D0.088070.000000.91193
333 A0.088070.000000.91193
334 H0.088070.000000.91193
335 V0.088070.000000.91193
336 A0.088070.000000.91193
337 G0.088070.000000.91193
338 P0.088070.000000.91193
339 D0.088070.000000.91193
340 H0.088070.000000.91193
341 Q0.088070.000000.91193
342 W0.088070.000000.91193
343 A0.088070.000000.91193
344 L0.088070.000000.91193
345 D0.088070.000000.91193
346 L0.088070.000000.91193
347 P0.088070.000000.91193
348 P0.088070.000000.91193
349 E0.088070.000000.91193
350 P0.088070.000000.91193
351 V0.088070.000000.91193
352 V0.088070.000000.91193
353 I0.088070.000000.91193
354 P0.088070.000000.91193
355 G0.088070.000000.91193
356 D0.088070.000000.91193
357 A0.088070.000000.91193
358 A0.088070.000000.91193
359 R0.088070.000000.91193
360 L0.088070.000000.91193
361 H0.088070.000000.91193
362 Q0.088070.000000.91193
363 V0.088060.000000.91193
364 V0.088060.000000.91193
365 T0.088060.000000.91193
366 N0.088060.000010.91193
367 L0.088060.000010.91193
368 L0.088060.000010.91193
369 A0.088060.000010.91193
370 N0.088060.000010.91193
371 A0.088060.000010.91193
372 R0.088060.000010.91193
373 V0.088060.000010.91193
374 H0.088060.000010.91193
375 T0.088060.000010.91193
376 G0.088050.000010.91193
377 P0.088050.000010.91193
378 G0.088050.000020.91193
379 T0.088050.000020.91193
380 I0.088050.000020.91193
381 V0.088050.000020.91193
382 T0.088050.000020.91193
383 T0.088050.000020.91193
384 R0.088050.000020.91193
385 L0.088050.000020.91193
386 S0.088050.000020.91193
387 T0.088050.000020.91193
388 G0.088050.000020.91193
389 P0.088050.000010.91193
390 T0.088050.000020.91193
391 H0.088040.000020.91193
392 V0.088040.000020.91193
393 V0.088040.000020.91193
394 L0.088040.000020.91193
395 Q0.088040.000020.91193
396 V0.088040.000020.91193
397 I0.088040.000020.91193
398 D0.088040.000010.91195
399 N0.088040.000010.91195
400 G0.088040.000010.91195
401 P0.088040.000010.91195
402 G0.088040.000010.91195
403 I0.088040.000010.91195
404 P0.088040.000010.91195
405 A0.088040.000010.91195
406 A0.088040.000010.91195
407 L0.088040.000010.91195
408 Q0.088050.000010.91195
409 S0.088050.000010.91195
410 E0.088050.000000.91195
411 V0.088050.000000.91195
412 F0.088050.000000.91195
413 E0.088050.000000.91195
414 R0.088050.000000.91195
415 F0.088050.000000.91195
416 A0.088050.000000.91195
417 R0.088050.000000.91195
418 G0.088050.000000.91195
419 D0.088050.000000.91195
420 T0.088040.000010.91195
421 S0.088020.000060.91192
422 R0.088010.000090.91189
423 S0.087920.000280.9118
424 R0.087840.000400.91176
425 Q0.087540.000860.9116
426 A0.086090.002400.91151
427 G0.084110.004410.91148
428 S0.083710.004820.91147
429 T0.083190.005370.91144
430 G0.081980.006620.9114
431 L0.081600.007020.91139
432 G0.081470.007150.91138
433 L0.081420.007210.91137
434 A0.081410.007230.91136
435 I0.081410.007230.91136
436 V0.081410.007230.91136
437 S0.081410.007230.91136
438 A0.081400.007230.91136
439 V0.081410.007230.91136
440 V0.081420.007200.91138
441 K0.081600.006880.91151
442 A0.081620.006850.91153
443 H0.081680.006730.91159
444 N0.081720.006570.91172
445 G0.081730.006490.91177
446 T0.081780.006280.91194
447 I0.081790.006040.91217
448 T0.081860.005160.91297
449 V0.081880.003920.9142
450 S0.081900.001630.91647
451 S0.081910.001140.91696
452 S0.081940.000770.91729
453 P0.081960.000280.91776
454 G0.081970.000150.91788
455 Y0.081980.000080.91794
456 T0.081990.000030.91798
457 E0.081990.000010.918
458 F0.081990.000010.918
459 A0.081990.000010.918
460 V0.081990.000010.918
461 R0.081990.000010.918
462 L0.081990.000010.918
463 P0.081990.000000.918
464 L0.081990.000000.918
465 D0.081990.000000.91801
466 G0.081990.000000.91801
467 W0.081990.000000.91801
468 Q0.081990.000000.91801
469 P0.081990.000000.91801
470 L0.081990.000000.91801
471 E0.081990.000000.91801
472 S0.081990.000000.91801
473 S0.081990.000000.91801
474 P0.081990.000000.91801
475 R0.081990.000000.91801

General Statistics
Length: 475
Number of predicted TMHs: 1
Exp number of AAs in TMHs: 24.75749
Exp number, first 60 AAs: 0.0027
Total prob of N-in: 0.84118
inside: from residue 1 to 152
TMhelix: from residue 153 to 175
outside: from residue 176 to 475
TMHMM predicts transmembrane helices and the location of the intervening loop regions.
If the whole sequence is labeled as inside or outside, the prediction is that it contains no membrane helices. It is probably not wise to interpret it as a prediction of location. The prediction gives the most probable location and orientation of transmembrane helices in the sequence.
Plot of probabilities
The plot shows the probabilities of inside/outside/TM helix. Possible weak TM helices are sometimes not predicted, and one can get an idea of the certainty of each segment in the prediction.
At the top of the plot (between 1 and 1.2) the best prediction is shown.
The plot is obtained by calculating the total probability that a residue sits in helix, inside, or outside summed over all possible paths through the model. Sometimes it seems like the plot and the prediction are contradictory, but that is because the plot shows probabilities for each residue, whereas the prediction is the over-all most probable structure. Therefore the plot should be seen as a complementary source of information.
Remarks
  • Predicted TM segments in the n-terminal region sometimes turn out to be signal peptides.
  • One of the most common mistakes by the program is to reverse the direction of proteins with one TM segment.
  • The program does not predict whether a non-membrane protein is cytoplasmic or not.
  • This prediction has been generated using TMHMM Server v. 2.0