TransMembrane prediction using Hidden Markov Models for Rv2394




Residue number
and Amino Acid
Probabilities
InsideMembraneOutside
1 V0.453320.000000.54668
2 S0.427260.026060.54668
3 V0.420820.032500.54668
4 W0.420820.032950.54623
5 L0.379670.074400.54593
6 R0.379670.074430.5459
7 A0.015640.438480.54588
8 G0.015630.438480.54588
9 A0.002040.452090.54588
10 L0.002020.452200.54579
11 V0.001670.452550.54578
12 A0.000920.453300.54578
13 A0.000420.453800.54578
14 V0.000420.453800.54578
15 M0.000420.453800.54578
16 L0.000410.453810.54578
17 S0.000410.453700.54588
18 L0.000410.453600.54598
19 S0.000410.453320.54626
20 G0.000410.450230.54936
21 C0.000410.447820.55177
22 G0.000440.445740.55381
23 G0.000470.444060.55547
24 F0.000530.442580.55689
25 H0.000620.413420.58597
26 A0.000640.407020.59234
27 G0.000950.348060.65099
28 A0.001200.292380.70642
29 P0.001260.246800.75195
30 S0.001280.005560.99315
31 T0.001290.004650.99406
32 A0.001290.001520.99719
33 G0.001310.000770.99792
34 P0.001310.000400.99828
35 C0.001310.000130.99856
36 E0.001310.000020.99867
37 I0.001310.000020.99867
38 V0.001310.000020.99867
39 P0.001310.000010.99868
40 N0.001310.000000.99868
41 G0.001310.000000.99869
42 T0.001310.000000.99869
43 P0.001310.000000.99869
44 A0.001310.000000.99869
45 P0.001310.000000.99869
46 K0.001310.000000.99869
47 T0.001310.000000.99869
48 P0.001310.000000.99869
49 P0.001310.000000.99869
50 A0.001310.000000.99869
51 T0.001310.000000.99869
52 V0.001310.000000.99869
53 P0.001310.000000.99869
54 S0.001310.000000.99869
55 S0.001310.000000.99869
56 R0.001310.000000.99869
57 N0.001310.000000.99869
58 L0.001310.000000.99869
59 A0.001310.000000.99869
60 T0.001310.000000.99869
61 N0.001310.000000.99869
62 P0.001310.000000.99869
63 E0.001310.000000.99869
64 I0.001310.000000.99869
65 A0.001310.000000.99869
66 T0.001310.000000.99869
67 G0.001310.000000.99869
68 Y0.001310.000000.99869
69 R0.001310.000000.99869
70 R0.001310.000000.99869
71 D0.001310.000000.99869
72 M0.001290.000030.99869
73 T0.001270.000050.99869
74 V0.001210.000100.99868
75 V0.001190.000140.99868
76 R0.001180.000150.99868
77 T0.001160.000170.99867
78 A0.001140.000200.99867
79 H0.001130.000200.99867
80 Y0.001130.000200.99867
81 A0.001130.000200.99867
82 A0.001130.000200.99867
83 A0.001130.000200.99867
84 T0.001130.000200.99867
85 A0.001130.000200.99867
86 N0.001130.000200.99867
87 P0.001130.000200.99867
88 L0.001130.000200.99867
89 A0.001130.000200.99867
90 T0.001130.000200.99867
91 Q0.001130.000190.99868
92 V0.001130.000180.99869
93 A0.001130.000160.99871
94 C0.001130.000100.99877
95 R0.001140.000060.99881
96 V0.001130.000050.99882
97 L0.001140.000040.99883
98 R0.001150.000010.99884
99 D0.001150.000010.99884
100 G0.001130.000020.99885
101 G0.001130.000030.99885
102 T0.001130.000030.99885
103 A0.001130.000030.99885
104 A0.001120.000030.99885
105 D0.001120.000030.99885
106 A0.001110.000040.99885
107 V0.001100.000070.99884
108 V0.001090.000080.99883
109 A0.001090.000100.99881
110 A0.001090.000110.9988
111 Q0.001080.000150.99877
112 A0.001060.000180.99876
113 V0.001040.000200.99876
114 L0.001030.000210.99876
115 G0.001030.000210.99876
116 L0.001030.000210.99876
117 V0.001030.000210.99876
118 E0.001030.000200.99877
119 P0.001030.000200.99877
120 Q0.001030.000200.99877
121 S0.001030.000200.99877
122 S0.001030.000200.99877
123 G0.001030.000190.99878
124 I0.001030.000190.99878
125 G0.001030.000190.99878
126 G0.001030.000180.99878
127 G0.001030.000180.99879
128 G0.001030.000180.99879
129 Y0.001030.000170.99879
130 L0.001040.000160.9988
131 V0.001040.000140.99881
132 Y0.001060.000120.99882
133 F0.001070.000100.99883
134 D0.001120.000020.99886
135 A0.001120.000010.99887
136 R0.001120.000000.99887
137 T0.001120.000000.99888
138 G0.001120.000000.99888
139 S0.001120.000000.99888
140 V0.001120.000000.99888
141 Q0.001120.000000.99888
142 A0.001120.000000.99888
143 Y0.001120.000000.99888
144 D0.001120.000000.99888
145 G0.001120.000000.99888
146 R0.001120.000000.99888
147 E0.001120.000000.99888
148 V0.001120.000000.99888
149 A0.001120.000000.99888
150 P0.001120.000000.99888
151 A0.001120.000000.99888
152 A0.001120.000000.99888
153 A0.001120.000000.99888
154 T0.001120.000000.99888
155 E0.001120.000000.99888
156 N0.001120.000000.99888
157 Y0.001120.000000.99888
158 L0.001120.000000.99888
159 R0.001120.000000.99888
160 W0.001120.000000.99888
161 V0.001120.000000.99888
162 S0.001120.000000.99888
163 D0.001120.000000.99888
164 V0.001120.000000.99888
165 D0.001120.000000.99888
166 R0.001120.000000.99888
167 S0.001120.000000.99888
168 A0.001120.000000.99888
169 P0.001120.000000.99888
170 R0.001120.000000.99888
171 P0.001120.000000.99888
172 N0.001120.000000.99888
173 A0.001120.000000.99888
174 R0.001120.000000.99888
175 A0.001120.000000.99888
176 S0.001120.000000.99888
177 G0.001120.000000.99888
178 R0.001120.000000.99888
179 S0.001120.000000.99888
180 I0.001120.000000.99888
181 G0.001120.000000.99888
182 V0.001120.000000.99888
183 P0.001120.000000.99888
184 G0.001120.000000.99888
185 I0.001120.000000.99888
186 L0.001120.000000.99888
187 R0.001120.000000.99888
188 M0.001120.000000.99888
189 L0.001120.000000.99888
190 E0.001120.000000.99888
191 M0.001120.000000.99888
192 V0.001120.000000.99888
193 H0.001120.000000.99888
194 N0.001120.000000.99888
195 E0.001120.000000.99888
196 H0.001120.000000.99888
197 G0.001120.000000.99888
198 R0.001120.000000.99888
199 T0.001120.000000.99888
200 P0.001120.000000.99888
201 W0.001120.000000.99888
202 R0.001120.000000.99888
203 D0.001120.000000.99888
204 L0.001120.000000.99888
205 F0.001120.000010.99888
206 G0.001120.000010.99888
207 P0.001120.000010.99888
208 A0.001120.000010.99888
209 V0.001120.000010.99888
210 T0.001120.000010.99888
211 L0.001120.000010.99888
212 A0.001120.000010.99888
213 D0.001120.000010.99888
214 G0.001120.000010.99888
215 G0.001120.000010.99888
216 F0.001120.000010.99888
217 D0.001120.000010.99888
218 I0.001120.000010.99888
219 S0.001120.000010.99888
220 A0.001120.000010.99888
221 R0.001120.000000.99888
222 M0.001120.000000.99888
223 G0.001120.000000.99888
224 A0.001120.000000.99888
225 A0.001120.000000.99888
226 I0.001120.000000.99888
227 S0.001120.000000.99888
228 D0.001120.000000.99888
229 A0.001120.000000.99888
230 A0.001120.000000.99888
231 P0.001120.000000.99888
232 Q0.001120.000000.99888
233 L0.001120.000000.99888
234 R0.001120.000000.99888
235 D0.001120.000000.99888
236 D0.001120.000000.99888
237 P0.001120.000000.99888
238 E0.001120.000000.99888
239 A0.001120.000000.99888
240 R0.001120.000000.99888
241 K0.001120.000000.99888
242 Y0.001120.000000.99888
243 F0.001120.000000.99888
244 L0.001120.000000.99888
245 N0.001120.000000.99888
246 P0.001120.000000.99888
247 D0.001120.000000.99888
248 G0.001120.000000.99888
249 S0.001120.000000.99888
250 P0.001120.000000.99888
251 K0.001120.000000.99888
252 P0.001120.000000.99888
253 A0.001120.000000.99888
254 G0.001120.000000.99888
255 T0.001120.000000.99888
256 R0.001120.000000.99888
257 L0.001120.000000.99888
258 T0.001120.000000.99888
259 N0.001120.000000.99888
260 P0.001110.000000.99888
261 A0.001110.000010.99888
262 Y0.001110.000010.99888
263 S0.001110.000010.99888
264 K0.001110.000010.99888
265 T0.001110.000020.99888
266 L0.001100.000030.99888
267 S0.001100.000030.99888
268 A0.001100.000030.99888
269 I0.001090.000030.99888
270 A0.001090.000030.99888
271 S0.001090.000030.99888
272 A0.001090.000030.99888
273 G0.001090.000030.99888
274 A0.001090.000030.99888
275 N0.001090.000030.99888
276 A0.001090.000030.99888
277 F0.001090.000030.99888
278 Y0.001090.000030.99888
279 S0.001100.000030.99888
280 G0.001100.000020.99888
281 D0.001100.000020.99888
282 I0.001100.000020.99888
283 A0.001100.000020.99888
284 H0.001100.000020.99889
285 D0.001100.000010.99889
286 I0.001100.000010.99889
287 V0.001100.000010.99889
288 A0.001100.000010.99889
289 A0.001100.000000.9989
290 A0.001100.000000.9989
291 S0.001100.000000.9989
292 D0.001100.000000.9989
293 T0.001100.000000.9989
294 S0.001100.000000.9989
295 N0.001100.000000.9989
296 G0.001100.000000.9989
297 R0.001100.000000.9989
298 T0.001100.000000.9989
299 P0.001100.000000.9989
300 G0.001100.000000.9989
301 L0.001100.000000.9989
302 L0.001100.000000.9989
303 T0.001100.000000.9989
304 I0.001100.000000.9989
305 E0.001100.000000.9989
306 D0.001100.000000.9989
307 L0.001100.000000.9989
308 A0.001100.000000.9989
309 G0.001100.000000.9989
310 Y0.001100.000000.9989
311 L0.001100.000000.9989
312 A0.001100.000000.9989
313 K0.001100.000000.9989
314 R0.001100.000000.9989
315 R0.001100.000000.9989
316 Q0.001100.000000.9989
317 P0.001100.000000.9989
318 L0.001100.000000.9989
319 C0.001100.000000.9989
320 T0.001100.000000.9989
321 T0.001090.000010.9989
322 Y0.001010.000090.9989
323 R0.000960.000140.9989
324 G0.000920.000180.9989
325 R0.000910.000190.99889
326 E0.000880.000230.99889
327 I0.000770.000370.99886
328 C0.000690.000450.99885
329 G0.000650.000500.99884
330 M0.000640.000520.99884
331 P0.000620.000550.99883
332 S0.000600.000570.99883
333 S0.000590.000590.99883
334 G0.000580.000590.99882
335 G0.000580.000590.99882
336 V0.000580.000590.99882
337 A0.000580.000590.99882
338 V0.000580.000590.99882
339 A0.000580.000590.99882
340 A0.000580.000590.99882
341 T0.000580.000590.99883
342 L0.000580.000580.99883
343 G0.000580.000580.99884
344 I0.000580.000560.99886
345 L0.000590.000440.99897
346 E0.000600.000300.9991
347 H0.000600.000270.99913
348 F0.000610.000250.99915
349 P0.000620.000190.99919
350 M0.000640.000120.99924
351 S0.000650.000040.9993
352 D0.000660.000010.99934
353 Y0.000660.000010.99934
354 A0.000660.000010.99934
355 P0.000660.000000.99934
356 S0.000660.000000.99935
357 K0.000660.000000.99935
358 V0.000660.000000.99935
359 D0.000660.000000.99935
360 L0.000660.000000.99935
361 N0.000660.000000.99935
362 G0.000660.000000.99935
363 G0.000660.000000.99935
364 R0.000660.000000.99935
365 P0.000660.000000.99935
366 T0.000660.000000.99935
367 V0.000660.000000.99935
368 M0.000660.000000.99935
369 G0.000660.000000.99935
370 V0.000660.000000.99935
371 H0.000660.000000.99935
372 L0.000660.000000.99935
373 I0.000660.000000.99935
374 A0.000660.000000.99935
375 E0.000660.000000.99935
376 A0.000660.000000.99935
377 E0.000660.000000.99935
378 R0.000660.000000.99935
379 L0.000660.000000.99935
380 A0.000660.000000.99935
381 Y0.000660.000000.99935
382 A0.000660.000000.99935
383 D0.000660.000000.99935
384 R0.000660.000000.99935
385 D0.000660.000000.99935
386 Q0.000660.000000.99935
387 Y0.000660.000000.99935
388 I0.000660.000000.99935
389 A0.000660.000000.99935
390 D0.000660.000000.99934
391 V0.000660.000000.99934
392 D0.000660.000000.99934
393 F0.000650.000010.99933
394 V0.000650.000010.99933
395 R0.000650.000020.99933
396 L0.000650.000020.99932
397 P0.000650.000020.99932
398 G0.000650.000030.99932
399 G0.000650.000030.99931
400 S0.000650.000030.99931
401 L0.000650.000030.99931
402 T0.000650.000030.99931
403 T0.000650.000040.99931
404 L0.000650.000040.99931
405 V0.000650.000040.99931
406 D0.000650.000040.99931
407 P0.000650.000040.99931
408 G0.000650.000040.99931
409 Y0.000650.000040.99931
410 L0.000650.000030.99931
411 A0.000650.000030.99931
412 A0.000650.000030.99931
413 R0.000650.000030.99931
414 A0.000650.000030.99931
415 A0.000650.000030.99931
416 L0.000660.000030.99932
417 I0.000660.000020.99932
418 S0.000680.000000.99932
419 P0.000680.000000.99932
420 Q0.000680.000000.99932
421 H0.000680.000000.99932
422 S0.000680.000000.99932
423 M0.000680.000000.99932
424 G0.000680.000000.99932
425 S0.000680.000000.99932
426 A0.000680.000000.99932
427 R0.000680.000000.99932
428 P0.000680.000000.99932
429 G0.000680.000000.99932
430 D0.000680.000000.99932
431 F0.000680.000000.99932
432 G0.000680.000000.99932
433 A0.000680.000000.99932
434 P0.000680.000000.99932
435 T0.000680.000000.99932
436 A0.000680.000000.99932
437 V0.000680.000000.99932
438 A0.000680.000000.99932
439 P0.000680.000000.99932
440 P0.000680.000000.99932
441 V0.000680.000000.99932
442 P0.000680.000000.99932
443 E0.000680.000000.99932
444 H0.000680.000000.99932
445 G0.000680.000000.99932
446 T0.000680.000000.99932
447 S0.000680.000000.99932
448 H0.000680.000000.99932
449 L0.000680.000000.99932
450 S0.000680.000000.99932
451 V0.000680.000000.99932
452 V0.000680.000000.99932
453 D0.000680.000000.99932
454 S0.000680.000000.99932
455 Y0.000680.000000.99932
456 G0.000680.000000.99932
457 N0.000680.000000.99932
458 A0.000680.000000.99931
459 A0.000680.000000.99931
460 T0.000680.000000.99931
461 L0.000680.000000.99931
462 T0.000680.000000.99931
463 T0.000680.000000.99931
464 T0.000680.000010.99931
465 V0.000680.000010.99931
466 E0.000680.000010.99931
467 S0.000680.000010.99931
468 S0.000680.000010.99931
469 F0.000680.000010.99931
470 G0.000680.000010.99931
471 S0.000680.000000.99931
472 Y0.000680.000000.99931
473 H0.000680.000000.99931
474 L0.000680.000000.99931
475 V0.000680.000000.99931
476 D0.000680.000000.99931
477 G0.000680.000000.99931
478 F0.000680.000000.99931
479 I0.000680.000000.99931
480 L0.000680.000000.99931
481 N0.000680.000000.99931
482 N0.000680.000000.99931
483 Q0.000680.000000.99931
484 L0.000680.000000.99931
485 S0.000680.000000.99931
486 D0.000680.000000.99931
487 F0.000680.000000.99931
488 S0.000680.000000.99931
489 A0.000680.000000.99931
490 E0.000680.000000.99931
491 P0.000680.000000.99931
492 H0.000680.000000.99931
493 A0.000680.000000.99931
494 T0.000680.000000.99931
495 D0.000680.000000.99931
496 G0.000680.000000.99931
497 S0.000680.000000.99931
498 P0.000680.000000.99931
499 V0.000680.000000.99931
500 A0.000680.000000.99931
501 N0.000680.000000.99931
502 R0.000680.000000.99931
503 V0.000680.000000.99931
504 E0.000680.000000.99931
505 P0.000680.000000.99931
506 G0.000680.000000.99931
507 K0.000680.000000.99931
508 R0.000680.000000.99931
509 P0.000680.000000.99931
510 R0.000680.000000.99931
511 S0.000680.000000.99931
512 S0.000680.000000.99931
513 M0.000680.000000.99931
514 A0.000680.000000.99931
515 P0.000680.000010.99931
516 T0.000680.000010.9993
517 L0.000680.000020.99929
518 V0.000680.000020.99929
519 F0.000680.000030.99929
520 D0.000680.000030.99928
521 H0.000680.000040.99928
522 S0.000670.000230.99909
523 S0.000650.001450.99789
524 A0.000630.003720.99565
525 G0.000620.003950.99543
526 R0.000610.004320.99506
527 G0.000520.009820.98966
528 A0.000460.013210.98632
529 L0.000390.015970.98364
530 Y0.000370.016250.98338
531 A0.000360.016840.9828
532 V0.000350.016980.98267
533 L0.000350.017020.98262
534 G0.000350.017070.98258
535 S0.000360.017130.98251
536 P0.000360.017220.98243
537 G0.000360.017260.98238
538 G0.000360.017290.98235
539 S0.000360.017370.98227
540 M0.000370.017450.98218
541 I0.000380.017470.98216
542 I0.000400.017440.98215
543 Q0.000680.017160.98216
544 F0.000710.017130.98216
545 V0.001010.016820.98217
546 V0.002970.014810.98221
547 K0.010440.007200.98236
548 T0.010740.006900.98237
549 L0.010880.006750.98237
550 V0.011850.005760.98238
551 A0.014090.003500.9824
552 M0.016190.001360.98244
553 L0.016390.001150.98246
554 D0.017060.000460.98248
555 W0.017080.000440.98248
556 G0.017110.000410.98248
557 L0.017140.000380.98249
558 N0.017320.000200.98249
559 P0.017450.000070.98249
560 Q0.017510.000010.98249
561 Q0.017510.000000.98249
562 A0.017510.000000.98249
563 V0.017510.000000.98249
564 S0.017510.000000.98249
565 L0.017510.000000.98249
566 V0.017510.000000.98249
567 D0.017510.000000.98249
568 F0.017510.000000.98249
569 G0.017510.000000.98249
570 A0.017510.000000.98249
571 A0.017510.000000.98249
572 N0.017510.000000.98249
573 S0.017510.000000.98249
574 P0.017510.000000.98249
575 H0.017510.000000.98249
576 T0.017510.000000.98249
577 N0.017510.000000.98249
578 L0.017510.000000.98249
579 G0.017510.000000.98249
580 G0.017510.000000.98249
581 E0.017510.000000.98249
582 N0.017510.000000.98249
583 P0.017510.000000.98249
584 E0.017510.000000.98249
585 I0.017510.000000.98249
586 N0.017510.000000.98249
587 T0.017510.000000.98249
588 S0.017510.000000.98249
589 D0.017510.000000.98249
590 D0.017510.000000.98249
591 G0.017510.000000.98249
592 D0.017510.000000.98249
593 H0.017510.000000.98249
594 D0.017510.000000.98249
595 P0.017510.000000.98249
596 L0.017510.000000.98249
597 V0.017510.000000.98249
598 Q0.017510.000000.98249
599 G0.017510.000000.98249
600 L0.017510.000000.98249
601 R0.017510.000000.98249
602 A0.017510.000000.98249
603 L0.017510.000000.98249
604 G0.017510.000000.98249
605 H0.017510.000000.98249
606 R0.017510.000000.98249
607 V0.017510.000000.98249
608 N0.017510.000000.98249
609 L0.017510.000000.98249
610 A0.017510.000000.98249
611 E0.017510.000000.98249
612 Q0.017510.000000.98249
613 S0.017510.000000.98249
614 S0.017510.000000.98249
615 G0.017510.000000.98249
616 L0.017510.000000.98249
617 S0.017510.000000.98249
618 A0.017510.000000.98249
619 I0.017510.000000.98249
620 T0.017510.000000.98249
621 R0.017510.000000.98249
622 S0.017510.000000.98249
623 E0.017510.000000.98249
624 A0.017510.000000.98249
625 G0.017510.000000.98249
626 W0.017510.000000.98249
627 A0.017510.000000.98249
628 G0.017510.000000.98249
629 G0.017510.000000.98249
630 A0.017510.000000.98249
631 D0.017510.000000.98249
632 P0.017510.000000.98249
633 R0.017510.000000.98249
634 R0.017510.000000.98249
635 E0.017510.000000.98249
636 G0.017510.000000.98249
637 A0.017510.000000.98249
638 V0.017510.000000.98249
639 M0.017510.000000.98249
640 G0.017510.000000.98249
641 D0.017510.000000.98249
642 D0.017510.000000.98249
643 A0.017510.000000.98249

General Statistics
Length: 643
Number of predicted TMHs: 0
Exp number of AAs in TMHs: 10.45348
Exp number, first 60 AAs: 10.05448
Total prob of N-in: 0.45332
POSSIBLE N-term signal sequence
outside: from residue 1 to 643
TMHMM predicts transmembrane helices and the location of the intervening loop regions.
If the whole sequence is labeled as inside or outside, the prediction is that it contains no membrane helices. It is probably not wise to interpret it as a prediction of location. The prediction gives the most probable location and orientation of transmembrane helices in the sequence.
Plot of probabilities
The plot shows the probabilities of inside/outside/TM helix. Possible weak TM helices are sometimes not predicted, and one can get an idea of the certainty of each segment in the prediction.
At the top of the plot (between 1 and 1.2) the best prediction is shown.
The plot is obtained by calculating the total probability that a residue sits in helix, inside, or outside summed over all possible paths through the model. Sometimes it seems like the plot and the prediction are contradictory, but that is because the plot shows probabilities for each residue, whereas the prediction is the over-all most probable structure. Therefore the plot should be seen as a complementary source of information.
Remarks
  • Predicted TM segments in the n-terminal region sometimes turn out to be signal peptides.
  • One of the most common mistakes by the program is to reverse the direction of proteins with one TM segment.
  • The program does not predict whether a non-membrane protein is cytoplasmic or not.
  • This prediction has been generated using TMHMM Server v. 2.0