TransMembrane prediction using Hidden Markov Models for Rv0020c




Residue number
and Amino Acid
Probabilities
InsideMembraneOutside
1 M0.006070.000000.99393
2 G0.006070.000000.99393
3 S0.006070.000000.99393
4 Q0.006070.000000.99393
5 K0.006070.000000.99393
6 R0.006070.000000.99393
7 L0.006070.000000.99393
8 V0.006070.000000.99393
9 Q0.006070.000000.99393
10 R0.006070.000000.99393
11 V0.006070.000000.99393
12 E0.006070.000000.99393
13 R0.006070.000000.99393
14 K0.006070.000000.99393
15 L0.006070.000000.99393
16 E0.006070.000000.99393
17 Q0.006070.000000.99393
18 T0.006070.000000.99393
19 V0.006060.000010.99393
20 G0.006060.000010.99393
21 D0.006040.000030.99393
22 A0.006030.000040.99393
23 F0.006020.000050.99393
24 A0.006020.000050.99393
25 R0.006020.000050.99393
26 I0.006020.000050.99393
27 F0.006020.000050.99393
28 G0.006020.000050.99393
29 G0.006020.000050.99393
30 S0.006020.000050.99393
31 I0.006020.000050.99393
32 V0.006020.000050.99393
33 P0.006020.000050.99393
34 Q0.006020.000050.99393
35 E0.006020.000050.99393
36 V0.006020.000050.99393
37 E0.006020.000040.99394
38 A0.006020.000040.99394
39 L0.006020.000030.99395
40 L0.006020.000020.99396
41 R0.006020.000010.99397
42 R0.006020.000000.99398
43 E0.006020.000000.99398
44 A0.006020.000000.99398
45 A0.006020.000000.99398
46 D0.006020.000000.99398
47 G0.006020.000000.99398
48 I0.006020.000000.99398
49 Q0.006020.000000.99398
50 S0.006020.000000.99398
51 L0.006010.000010.99398
52 Q0.006010.000010.99398
53 G0.006010.000010.99398
54 N0.006010.000010.99398
55 R0.006010.000010.99398
56 L0.006010.000010.99398
57 L0.006010.000010.99398
58 A0.006010.000010.99398
59 P0.006010.000010.99398
60 N0.006010.000010.99398
61 E0.006010.000010.99398
62 Y0.006010.000010.99398
63 I0.006010.000010.99398
64 I0.006010.000010.99398
65 T0.006010.000010.99398
66 L0.006010.000010.99398
67 G0.006010.000010.99398
68 V0.006010.000010.99398
69 H0.006010.000010.99398
70 D0.006010.000000.99399
71 F0.006010.000000.99399
72 E0.006010.000000.99399
73 K0.006010.000000.99399
74 L0.006010.000000.99399
75 G0.006010.000000.99399
76 A0.006010.000000.99399
77 D0.006010.000000.99399
78 P0.006010.000000.99399
79 E0.006010.000000.99399
80 L0.006010.000000.99399
81 K0.006010.000000.99399
82 S0.006010.000000.99399
83 T0.006010.000000.99399
84 G0.006010.000000.99399
85 F0.006010.000000.99399
86 A0.006010.000000.99399
87 R0.006010.000000.99399
88 D0.006010.000000.99399
89 L0.006010.000000.99399
90 A0.006010.000000.99399
91 D0.006010.000000.99399
92 Y0.006010.000000.99399
93 I0.006010.000000.99399
94 Q0.006010.000000.99399
95 E0.006010.000000.99399
96 Q0.006010.000000.99399
97 G0.006010.000000.99399
98 W0.006010.000000.99399
99 Q0.006010.000000.99399
100 T0.006010.000000.99399
101 Y0.006010.000000.99399
102 G0.006010.000000.99399
103 D0.006010.000000.99399
104 V0.006010.000000.99399
105 V0.006010.000000.99399
106 V0.006010.000000.99399
107 R0.006010.000000.99399
108 F0.006010.000000.99399
109 E0.006010.000000.99399
110 Q0.006010.000000.99399
111 S0.006010.000000.99399
112 S0.006010.000000.99399
113 N0.006010.000000.99399
114 L0.006010.000000.99399
115 H0.006010.000000.99399
116 T0.006010.000000.99399
117 G0.006010.000000.99399
118 Q0.006010.000000.99399
119 F0.006010.000000.99399
120 R0.006010.000000.99399
121 A0.006010.000000.99399
122 R0.006010.000000.99399
123 G0.006010.000000.99399
124 T0.006010.000000.99399
125 V0.006010.000000.99399
126 N0.006010.000000.99399
127 P0.006010.000000.99399
128 D0.006010.000000.99399
129 V0.006010.000000.99399
130 E0.006010.000000.99399
131 T0.006010.000000.99399
132 H0.006010.000000.99399
133 P0.006010.000000.99399
134 P0.006010.000000.99399
135 V0.006010.000000.99399
136 I0.006010.000000.99399
137 D0.006010.000000.99399
138 C0.006010.000000.99399
139 A0.006010.000000.99399
140 R0.006010.000000.99399
141 P0.006010.000000.99399
142 Q0.006010.000000.99399
143 S0.006010.000000.99399
144 N0.006010.000000.99399
145 H0.006010.000000.99399
146 A0.006010.000000.99399
147 F0.006010.000000.99399
148 G0.006010.000000.99399
149 A0.006010.000000.99399
150 E0.006010.000000.99399
151 P0.006010.000000.99399
152 G0.006010.000000.99399
153 V0.006010.000000.99399
154 A0.006010.000000.99399
155 P0.006010.000000.99399
156 M0.006010.000000.99399
157 S0.006010.000000.99399
158 D0.006010.000000.99399
159 N0.006010.000000.99399
160 S0.006010.000000.99399
161 S0.006010.000000.99399
162 Y0.006010.000000.99399
163 R0.006010.000000.99399
164 G0.006010.000000.99399
165 G0.006010.000000.99399
166 Q0.006010.000000.99399
167 G0.006010.000000.99399
168 Q0.006010.000000.99399
169 G0.006010.000000.99399
170 R0.006010.000000.99399
171 P0.006010.000000.99399
172 D0.006010.000000.99399
173 E0.006010.000000.99399
174 Y0.006010.000000.99399
175 Y0.006010.000000.99399
176 D0.006010.000000.99399
177 D0.006010.000000.99399
178 R0.006010.000000.99399
179 Y0.006010.000000.99399
180 A0.006010.000000.99399
181 R0.006010.000000.99399
182 P0.006010.000000.99399
183 Q0.006010.000000.99399
184 E0.006010.000000.99399
185 D0.006010.000000.99399
186 P0.006010.000000.99399
187 R0.006010.000000.99399
188 G0.006010.000000.99399
189 G0.006010.000000.99399
190 P0.006010.000000.99399
191 D0.006010.000000.99399
192 P0.006010.000000.99399
193 Q0.006010.000000.99399
194 G0.006010.000000.99399
195 G0.006010.000000.99399
196 S0.006010.000000.99399
197 D0.006010.000000.99399
198 P0.006010.000000.99399
199 R0.006010.000000.99399
200 G0.006010.000000.99399
201 G0.006010.000000.99399
202 Y0.006010.000000.99399
203 P0.006010.000000.99399
204 P0.006010.000000.99399
205 E0.006010.000000.99399
206 T0.006010.000000.99399
207 G0.006010.000000.99399
208 G0.006010.000000.99399
209 Y0.006010.000000.99399
210 P0.006010.000000.99399
211 P0.006010.000000.99399
212 Q0.006010.000000.99399
213 P0.006010.000000.99399
214 G0.006010.000000.99399
215 Y0.006010.000000.99399
216 P0.006010.000000.99399
217 R0.006010.000000.99399
218 P0.006010.000000.99399
219 R0.006010.000000.99399
220 H0.006010.000000.99399
221 P0.006010.000000.99399
222 D0.006010.000000.99399
223 Q0.006010.000000.99399
224 G0.006010.000000.99399
225 D0.006010.000000.99399
226 Y0.006010.000000.99399
227 P0.006010.000000.99399
228 E0.006010.000000.99399
229 Q0.006010.000000.99399
230 I0.006010.000000.99399
231 G0.006010.000000.99399
232 Y0.006010.000000.99399
233 P0.006010.000000.99399
234 D0.006010.000000.99399
235 Q0.006010.000000.99399
236 G0.006010.000000.99399
237 G0.006010.000000.99399
238 Y0.006010.000000.99399
239 P0.006010.000000.99399
240 E0.006010.000000.99399
241 Q0.006010.000000.99399
242 R0.006010.000000.99399
243 G0.006010.000000.99399
244 Y0.006010.000000.99399
245 P0.006010.000000.99399
246 E0.006010.000000.99399
247 Q0.006010.000000.99399
248 R0.006010.000000.99399
249 G0.006010.000000.99399
250 Y0.006010.000000.99399
251 P0.006010.000000.99399
252 D0.006010.000000.99399
253 Q0.006010.000000.99399
254 R0.006010.000000.99399
255 G0.006010.000000.99399
256 Y0.006010.000000.99399
257 Q0.006010.000000.99399
258 D0.006010.000000.99399
259 Q0.006010.000000.99399
260 G0.006010.000000.99399
261 R0.006010.000000.99399
262 G0.006010.000000.99399
263 Y0.006010.000000.99399
264 P0.006010.000000.99399
265 D0.006010.000000.99399
266 Q0.006010.000000.99399
267 G0.006010.000000.99399
268 Q0.006010.000000.99399
269 G0.006010.000000.99399
270 G0.006010.000000.99399
271 Y0.006010.000000.99399
272 P0.006010.000000.99399
273 P0.006010.000000.99399
274 P0.006010.000000.99399
275 Y0.006010.000000.99399
276 E0.006010.000000.99399
277 Q0.006010.000000.99399
278 R0.006010.000000.99399
279 P0.006010.000000.99399
280 P0.006010.000000.99399
281 V0.006010.000000.99399
282 S0.006010.000000.99399
283 P0.006010.000000.99399
284 G0.006010.000000.99399
285 P0.006010.000000.99399
286 A0.006010.000000.99399
287 A0.006010.000000.99399
288 G0.006010.000000.99399
289 Y0.006010.000000.99399
290 G0.006010.000000.99399
291 A0.006010.000000.99399
292 P0.006010.000000.99399
293 G0.006010.000000.99399
294 Y0.006010.000000.99399
295 D0.006010.000000.99399
296 Q0.006010.000000.99399
297 G0.006010.000000.99399
298 Y0.006010.000000.99399
299 R0.006010.000000.99399
300 Q0.006010.000000.99399
301 S0.006010.000000.99399
302 G0.006010.000000.99399
303 G0.006010.000000.99399
304 Y0.006010.000000.99399
305 G0.006010.000000.99399
306 P0.006010.000000.99399
307 S0.006010.000000.99399
308 P0.006010.000000.99399
309 G0.006010.000000.99399
310 G0.006010.000000.99399
311 G0.006010.000000.99399
312 Q0.006010.000000.99399
313 P0.006010.000000.99399
314 G0.006010.000000.99399
315 Y0.006010.000000.99399
316 G0.006010.000000.99399
317 G0.006010.000000.99399
318 Y0.006010.000000.99399
319 G0.006010.000000.99399
320 E0.006010.000000.99399
321 Y0.006010.000000.99399
322 G0.006010.000000.99399
323 R0.006010.000000.99399
324 G0.006010.000000.99399
325 P0.006010.000000.99399
326 A0.006010.000000.99399
327 R0.006010.000000.99399
328 H0.006010.000000.99399
329 E0.006010.000000.99399
330 E0.006010.000000.99399
331 G0.006010.000000.99399
332 S0.006010.000000.99399
333 Y0.006010.000000.99399
334 V0.006010.000000.99399
335 P0.006010.000000.99399
336 S0.006010.000000.99399
337 G0.006010.000000.99399
338 P0.006010.000000.99399
339 P0.006010.000000.99399
340 G0.006010.000000.99399
341 P0.006010.000000.99399
342 P0.006010.000000.99399
343 E0.006010.000000.99399
344 Q0.006010.000000.99399
345 R0.006010.000000.99399
346 P0.006010.000000.99399
347 A0.006010.000000.99399
348 Y0.006010.000000.99399
349 P0.006010.000000.99399
350 D0.006010.000000.99399
351 Q0.006010.000000.99399
352 G0.006010.000000.99399
353 G0.006010.000000.99399
354 Y0.006010.000000.99399
355 D0.006010.000000.99399
356 Q0.006010.000000.99399
357 G0.006010.000000.99399
358 Y0.006010.000000.99399
359 Q0.006010.000000.99399
360 Q0.006010.000000.99399
361 G0.006010.000000.99399
362 A0.006010.000000.99399
363 T0.006010.000000.99399
364 T0.006010.000000.99399
365 Y0.006010.000000.99399
366 G0.006010.000000.99399
367 R0.006010.000000.99399
368 Q0.006010.000000.99399
369 D0.006010.000000.99399
370 Y0.006010.000000.99399
371 G0.006010.000000.99399
372 G0.006010.000000.99399
373 G0.006010.000000.99399
374 A0.006010.000000.99399
375 D0.006010.000000.99399
376 Y0.006010.000000.99399
377 T0.006010.000000.99399
378 R0.006010.000000.99399
379 Y0.006010.000000.99399
380 T0.006010.000000.99399
381 E0.006010.000000.99399
382 S0.006010.000000.99399
383 P0.006010.000000.99399
384 R0.006010.000000.99399
385 V0.006010.000000.99399
386 P0.006010.000000.99399
387 G0.006010.000000.99399
388 Y0.006010.000000.99399
389 A0.006010.000000.99399
390 P0.006010.000000.99399
391 Q0.006010.000000.99399
392 G0.006010.000000.99399
393 G0.006010.000000.99399
394 G0.006010.000000.99399
395 Y0.006010.000000.99399
396 A0.006010.000000.99399
397 E0.006010.000000.99399
398 P0.006010.000000.99399
399 A0.006010.000000.99399
400 G0.006010.000000.99399
401 R0.006010.000000.99399
402 D0.006010.000000.99399
403 Y0.006010.000000.99399
404 D0.006010.000000.99399
405 Y0.006010.000000.99399
406 G0.006010.000000.99399
407 Q0.006010.000000.99399
408 S0.006010.000000.99399
409 G0.006010.000000.99399
410 A0.006010.000000.99399
411 P0.006010.000000.99399
412 D0.006010.000000.99399
413 Y0.006010.000000.99399
414 G0.006010.000000.99399
415 Q0.006010.000000.99399
416 P0.006010.000000.99399
417 A0.006010.000000.99399
418 P0.006010.000000.99399
419 G0.006010.000000.99399
420 G0.006010.000000.99399
421 Y0.006010.000000.99399
422 S0.006010.000000.99398
423 G0.006010.000010.99398
424 Y0.006010.000010.99398
425 G0.006010.000010.99398
426 Q0.006010.000010.99398
427 G0.006010.000010.99398
428 G0.006010.000010.99398
429 Y0.006010.000010.99398
430 G0.006010.000010.99398
431 S0.006010.000010.99398
432 A0.006010.000010.99398
433 G0.006010.000010.99398
434 T0.006010.000010.99398
435 S0.006010.000010.99398
436 V0.006010.000010.99398
437 T0.006010.000010.99398
438 L0.006010.000010.99398
439 Q0.006010.000000.99398
440 L0.006010.000000.99398
441 D0.006020.000000.99398
442 D0.006020.000000.99398
443 G0.006020.000000.99398
444 S0.006020.000000.99398
445 G0.006020.000000.99398
446 R0.006020.000000.99398
447 T0.006020.000000.99398
448 Y0.006020.000000.99398
449 Q0.006020.000000.99398
450 L0.006020.000000.99398
451 R0.006020.000000.99398
452 E0.006020.000000.99398
453 G0.006020.000000.99398
454 S0.006020.000000.99398
455 N0.006020.000000.99398
456 I0.006020.000000.99398
457 I0.006020.000000.99398
458 G0.006020.000000.99398
459 R0.006020.000000.99398
460 G0.006020.000000.99398
461 Q0.006020.000000.99398
462 D0.006020.000000.99398
463 A0.006020.000000.99398
464 Q0.006020.000000.99398
465 F0.006020.000000.99398
466 R0.006020.000000.99398
467 L0.006020.000000.99398
468 P0.006020.000000.99398
469 D0.006020.000000.99398
470 T0.006020.000000.99398
471 G0.006020.000000.99398
472 V0.006020.000000.99398
473 S0.006020.000000.99398
474 R0.006020.000000.99398
475 R0.006020.000000.99398
476 H0.006020.000000.99398
477 L0.006020.000000.99398
478 E0.006020.000000.99398
479 I0.006020.000000.99398
480 R0.006020.000000.99398
481 W0.006020.000000.99398
482 D0.006020.000000.99398
483 G0.006020.000000.99398
484 Q0.006020.000000.99398
485 V0.006020.000000.99398
486 A0.006020.000000.99398
487 L0.006020.000000.99398
488 L0.006020.000000.99398
489 A0.006020.000000.99398
490 D0.006020.000000.99398
491 L0.006020.000000.99398
492 N0.006020.000000.99398
493 S0.006020.000000.99398
494 T0.006020.000000.99398
495 N0.006020.000000.99398
496 G0.006020.000000.99398
497 T0.006020.000000.99398
498 T0.006020.000000.99398
499 V0.006020.000000.99398
500 N0.006020.000000.99398
501 N0.006020.000000.99398
502 A0.006020.000000.99398
503 P0.006020.000000.99398
504 V0.006020.000000.99398
505 Q0.006020.000000.99398
506 E0.006020.000000.99398
507 W0.006020.000000.99398
508 Q0.006020.000000.99398
509 L0.006020.000000.99398
510 A0.006020.000000.99398
511 D0.006020.000000.99398
512 G0.006020.000000.99398
513 D0.006020.000000.99398
514 V0.006020.000000.99398
515 I0.006020.000000.99398
516 R0.006020.000000.99398
517 L0.006020.000000.99398
518 G0.006020.000000.99398
519 H0.006020.000000.99398
520 S0.006020.000000.99398
521 E0.006020.000000.99398
522 I0.006020.000000.99398
523 I0.006020.000000.99398
524 V0.006020.000000.99398
525 R0.006020.000000.99398
526 M0.006020.000000.99398
527 H0.006020.000000.99398

General Statistics
Length: 527
Number of predicted TMHs: 0
Exp number of AAs in TMHs: 0.00128
Exp number, first 60 AAs: 0.00103
Total prob of N-in: 0.00607
outside: from residue 1 to 527
TMHMM predicts transmembrane helices and the location of the intervening loop regions.
If the whole sequence is labeled as inside or outside, the prediction is that it contains no membrane helices. It is probably not wise to interpret it as a prediction of location. The prediction gives the most probable location and orientation of transmembrane helices in the sequence.
Plot of probabilities
The plot shows the probabilities of inside/outside/TM helix. Possible weak TM helices are sometimes not predicted, and one can get an idea of the certainty of each segment in the prediction.
At the top of the plot (between 1 and 1.2) the best prediction is shown.
The plot is obtained by calculating the total probability that a residue sits in helix, inside, or outside summed over all possible paths through the model. Sometimes it seems like the plot and the prediction are contradictory, but that is because the plot shows probabilities for each residue, whereas the prediction is the over-all most probable structure. Therefore the plot should be seen as a complementary source of information.
Remarks
  • Predicted TM segments in the n-terminal region sometimes turn out to be signal peptides.
  • One of the most common mistakes by the program is to reverse the direction of proteins with one TM segment.
  • The program does not predict whether a non-membrane protein is cytoplasmic or not.
  • This prediction has been generated using TMHMM Server v. 2.0