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Gene: recF

General annotation | Coordinates | Sequence | Genome Browser | Structural information | Orthologs/Cross-references | Interacting Drugs/Compounds | Bibliography


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General annotation
Gene namerecF
Rv numberRv0003
TypeCDS
FunctionTHE RECF PROTEIN IS INVOLVED IN DNA METABOLISM AND RECOMBINATION; IT IS REQUIRED FOR DNA REPLICATION AND NORMAL SOS INDUCIBILITY. RECF BINDS PREFERENTIALLY TO SINGLE-STRANDED, LINEAR DNA. IT ALSO SEEMS TO BIND ATP.
ProductDNA REPLICATION AND REPAIR PROTEIN RECF (SINGLE-STRAND DNA BINDING PROTEIN)
Evidenceexperimental
CommentsRv0003, (MTCY10H4.01), len: 385 aa. recF, DNA replication and repair protein (see citations below), equivalent to others Mycobacterial DNA replication and repair proteins e.g. NP_301131.1|NC_002677 from Mycobacterium leprae (385 aa); Q9L7L5|RECF_MYCPA from Mycobacterium avium subsp. paratuberculosis (385 aa); P50916|RECF_MYCSM from Mycobacterium smegmatis (384 aa); etc. Also highly similar to others e.g. P36176|RECF_STRCO DNA REPLICATION AND REPAIR PROTEIN from Streptomyces coelicolor (373 aa); NP_440892.1|NC_000911 from Synechocystis sp. strain PCC 6803 (384 aa); NP_469352.1|NC_003212 from Listeria innocua (370 aa); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00617 RecF protein signature 1, and PS00618 RecF protein signature 2. BELONGS TO THE RECF FAMILY.
Molecular mass (Da)42180.1
Isoelectric point7.201
Gene length (bp)1158
Protein length385
Location (kb)3.28


Functional categoryinformation pathways


ProteomicsIdentified in the cytosol and cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005).
TranscriptomemRNA identified by microarray analysis and down-regulated after 96h of starvation (see Betts et al., 2002).
Mutationnon essential gene by Himar1-based transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003) mutants available at TARGET website
RegulonPredicted to be in the IdeR|Rv2711 regulon (See Gold et al., 2001).
Annotation Changesregulon, bibliography updated on 01-JUL-2009


Coordinates
TypeStartEndOrientation
CDS32804437+


Protein sequence in FASTA format
>M. tuberculosis H37Rv | recF
MYVRHLGLRDFRSWACVDLELHPGRTVFVGPNGYGKTNLIEALWYSTTLGSHRVSADLPLIRVGTDRAVISTIVVNDGRE
CAVDLEIATGRVNKARLNRSSVRSTRDVVGVLRAVLFAPEDLGLVRGDPADRRRYLDDLAIVRRPAIAAVRAEYERVLRQ
RTALLKSVPGARYRGDRGVFDTLEVWDSRLAEHGAELVAARIDLVNQLAPEVKKAYQLLAPESRSASIGYRASMDVTGPS
EQSDIDRQLLAARLLAALAARRDAELERGVCLVGPHRDDLILRLGDQPAKGFASHGEAWSLAVALRLAAYQLLRVDGGEP
VLLLDDVFAELDVMRRRALATAAESAEQVLVTAAVLEDIPAGWDARRVHIDVRADDTGSMSVVLP
Blastp: results
TransMembrane prediction using Hidden Markov Models: tmhmm
Microbe Genome Browser
Genomic sequence

Add extra bases upstream (5') and downstream (3')



Structural information
Protein Data BankNo structure available
PFAMProtein Family Domains


Orthologs/Cross-references
CDC1551MT0003
Gene Ontologysingle-stranded DNA binding
ATP binding
cytoplasm
DNA replication
DNA repair
SOS response
MbovisMb0003
MlepraeML0003
MsmegmatisMSMEG_0003
UniProtQ59586


Interacting Drugs/Compounds
TDR TargetsRv0003


Bibliography
Qin MH, Madiraju MV, Rajagopalan M,
Characterization of the functional replication origin of Mycobacterium tuberculosis
Gene (1999) 233 (1-2):121-30
Gold B, Rodriguez GM, Marras SA, Pentecost M, Smith I,
The Mycobacterium tuberculosis IdeR is a dual functional regulator that controls transcription of genes involved in iron acquisition, iron storage and survival in macrophages.
Mol Microbiol (2001) 42(3):851-65
Betts JC, Lukey PT, Robb LC, McAdam RA, Duncan K,
Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling
Mol Microbiol (2002) 43 :717
Sassetti CM, Boyd DH, Rubin EJ,
Genes required for mycobacterial growth defined by high density mutagenesis.
Mol Microbiol (2003) 48(1):77-84
Lamichhane G, Zignol M, Blades NJ, Geiman DE, Dougherty A, Grosset J, Broman KW, Bishai WR,
A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis.
Proc Natl Acad Sci U S A (2003) 100 :7213
Mawuenyega KG, Forst CV, Dobos KM, Belisle JT, Chen J, Bradbury EM, Bradbury AR, Chen X,
Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling.
Mol Biol Cell (2005) 16(1):396-404
Salazar L, Fsihi H, De Rossi E, Riccardi G, Rios C, Cole ST, Takiff HE,
Organization of the origins of replication of the chromosomes of Mycobacterium smegmatis, Mycobacterium leprae and Mycobacterium tuberculosis and isolation of a functional origin from Mycobacterium smegmatis
Mol Microbiol (1996) 20(2):283-93
Mizrahi V, Andersen SJ,
DNA repair in Mycobacterium tuberculosis. What have we learnt from the genome sequence?
Mol Microbiol (1998) 29(6):1331-9