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Gene: fadD34

General annotation | Coordinates | Sequence | Genome Browser | Structural information | Orthologs/Cross-references | Interacting Drugs/Compounds | Bibliography


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General annotation
Gene namefadD34
Rv numberRv0035
TypeCDS
FunctionFUNCTION UNKNOWN, BUT INVOLVED IN LIPID DEGRADATION.
ProductPROBABLE FATTY-ACID-CoA LIGASE FADD34 (FATTY-ACID-CoA SYNTHETASE) (FATTY-ACID-CoA SYNTHASE)
CommentsRv0035, (MTCY10H4.35), len: 562 aa. Probable fadD34, fatty-acid-CoA synthetase , similar to many e.g. MBU75685_1 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA scores: opt: 408, E(): 8.2e-20; etc. Also similar to G1171128 SAFRAMYCIN MX1 SYNTHETASE B (1770 aa), FASTA scores: opt: 445, E(): 1.3e-21, (28.1% identity in 573 aa overlap). Also similar to other proteins from Mycobacterium tuberculosis e.g. MTCY02B10.09, FASTA score: (32.3% identity in 468 aa overlap), MTCY349_40, MTCY4D9_17, MTCY338_18, MTV045_3, MTCY409_4, MTCI237_30, MTCY24G1_8, MASC_MYCLE MASC PROTEIN, U00010_6, MTV005_21, MTCY19G5_7, MTCY9F9_39, etc.
Molecular mass (Da)60961.4
Isoelectric point6.4656
Gene length (bp)1689
Protein length562
Location (kb)37.259


Functional categorylipid metabolism


Mutationnon essential gene by Himar1-based transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003) mutants available at TARGET website


Coordinates
TypeStartEndOrientation
CDS3725938947+


Protein sequence in FASTA format
>M. tuberculosis H37Rv | fadD34
MTAALLSPAIAWQQISACTDRTLTITCEDSEVISYQDLIARAAACIPPLRRLDLKRGEPVLITAHTNLEFLSCFLGLMLH
GAVPVPIPPREALKTTERFMTRLGPLLRHHRVLICTPAEHDEIRAAASTDCQISRFTALAEAGDEQFGRATAQQLADTAT
ADWPLCTLDDDAYVQYTSGSTAAPRGVVITYRNLLSNMRAMAVGSQFQHGDVMGSWLPLHHDMGLVGSLFAALFNSVSAV
FTTPHRFLYDPLGFLRLLTSSGATHTFMPNFALEWLINAYHRRGADIEGIDLHKMRRLIIASEPVHAEGMRRFAATFAGV
GLAPTALGSGYGLAEATVAVSMSAPNTGFRTETHAAAEVVTGGRVLPGYEVRIDAAPGARAGTIKLRGDSVAAKAYVGGK
KLDALDEEGFCDTHDLGFLVDDEIVILGRQDEVFIVHGENRFPYDIEFIIRGESEQHRTKVACFGVNERVVVVLESPLDS
IIDKAEADRLRCQVVAATGLQLDELITVRRGAIPTTTSGKLKRRAVAQAYRDGTLPRLATHAWTADPDSAPKTTRSSLEG
AH
Blastp: results
TransMembrane prediction using Hidden Markov Models: tmhmm
Microbe Genome Browser
Genomic sequence

Add extra bases upstream (5') and downstream (3')



Structural information
Protein Data BankNo structure available
PFAMProtein Family Domains


Orthologs/Cross-references
CDC1551MT0040
Enzyme Classification6.2.1.-
Gene Ontologymetabolic process
ligase activity
MbovisMb0036
MmarinumMMAR_4970
UniProtQ7DAJ8


Interacting Drugs/Compounds
TDR TargetsRv0035


Expression Data
TBDBRv0035


Bibliography
Sassetti CM, Boyd DH, Rubin EJ,
Genes required for mycobacterial growth defined by high density mutagenesis.
Mol Microbiol (2003) 48(1):77-84
Lamichhane G, Zignol M, Blades NJ, Geiman DE, Dougherty A, Grosset J, Broman KW, Bishai WR,
A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis.
Proc Natl Acad Sci U S A (2003) 100 :7213