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Search term: Rv3266c

General annotation | Coordinates | Sequence | Genome Browser | Structural information | Orthologs/Cross-references | Interacting Drugs/Compounds | Bibliography


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General annotation
Gene namermlD
Rv numberRv3266c
TypeCDS
FunctionINVOLVED IN dTDP-L-RHAMNOSE BIOSYNTHESIS: CONVERTS dTDP-6-DEOXY-L-LYXO-4-HEXULOSE TO dTDP-L-RHAMNOSE WITH THE CONCOMITANT OXIDATION OF NADPH TO NADP+ [CATALYTIC ACTIVITY: dTDP-6-DEOXY-L-LYXO-4-HEXULOSE + NADPH = dTDP-L-RHAMNOSE + NADP+].
ProductdTDP-6-DEOXY-L-LYXO-4-HEXULOSE REDUCTASE RMLD (dTDP-RHAMNOSE MODIFICATION PROTEIN) (dTDP-RHAMNOSE BIOSYNTHESIS PROTEIN) (dTDP-RHAMNOSE SYNTHASE)
Evidenceexperimental
CommentsRv3266c, (MTCY71.06c), len: 304 aa. rmlD, dTDP-6-deoxy-L-lyxo-4-hexulose reductase (dTDP-rhamnose modification protein) (see citations below), highly similar to Q9CCK8 putative dTDP-rhamnose modification protein from Mycobacterium leprae (311 aa), FASTA scores, opt: 1440, E(): 1.1e-78, (74.7% identity in 312 aa overlap); and similar to several dTDP-4-dehydrorhamnose reductase e.g. STRL_STRGR|P29781 from Streptomyces griseus (304 aa), FASTA scores, opt: 788, E(): 0, (47.4% identity in 304 aa overlap).
Molecular mass (Da)32044.8
Isoelectric point6.8824
Gene length (bp)915
Protein length304
Location (kb)3646.9


Functional categoryintermediary metabolism and respiration


MutationM. smegmatis knockout shows rmlD is essential for growth (See Ma et al., 2002). mutants available at TARGET website
Annotation ChangesMutation, Bibliography, updated 01-OCT-2008


Coordinates
TypeStartEndOrientation
CDS36468953647809-


Protein sequence in FASTA format
>M. tuberculosis H37Rv | rmlD
MAGRSERLVITGAGGQLGSHLTAQAAREGRDMLALTSSQWDITDPAAAERIIRHGDVVINCAAYTDVDGAESNEAVAYAV
NATGPQHLARACARVGARLIHVSTDYVFDGDFGGAEPRPYEPTDETAPQGVYARSKLAGEQAVLAAFPEAAVVRTAWVYT
GGTGKDFVAVMRRLAAGHGRVDVVDDQTGSPTYVADLAEALLALADAGVRGRVLHAANEGVVSRFGQARAVFEECGADPQ
RVRPVSSAQFPRPAPRSSYSALSSRQWALAGLTPLRHWRSALATALAAPANSTSIDRRLPSTRD
Blastp: results
TransMembrane prediction using Hidden Markov Models: tmhmm
Microbe Genome Browser
Genomic sequence

Add extra bases upstream (5') and downstream (3')



Structural information
Protein Data BankNo structure available
PFAMP96871


Orthologs/Cross-references
CDC1551MT3366
Enzyme Classification1.1.1.133
Gene OntologydTDP-4-dehydrorhamnose reductase activity
extracellular polysaccharide biosynthetic process
oxidation reduction
MbovisMb3294c
MlepraeML0751
MmarinumMMAR_1275
MsmegmatisMSMEG_1825
UniProtP96871
Multiple Sequences Alignment: between orthologs


Interacting Drugs/Compounds
TDR TargetsRv3266c
Drug Resistance MutationsEthambutol


Expression Data
TBDBRv3266c


Bibliography
Hoang TT, Ma Y, Stern RJ, McNeil MR, Schweizer HP,
Construction and use of low-copy number T7 expression vectors for purification of problem proteins: purification of mycobacterium tuberculosis RmlD and pseudomonas aeruginosa LasI and RhlI proteins, and functional analysis of purified RhlI
Gene (1999) 237(2):361-71
Giraud MF, McMiken HJ, Leonard GA, Messner P, Whitfield C, Naismith JH,
Overexpression , purification, crystallization and preliminary structural study of dTDP-6-deoxy-L-lyxo-4-hexulose reductase (RmlD), the fourth enzyme of the dTDP-L-rhamnose synthesis pathway , from Salmonella enterica serovar Typhimurium
Acta Crystallogr D Biol Crystallogr (1999) 55(12):2043-6
Ma Y, Stern RJ, Scherman MS, Vissa VD, Yan W, Jones VC, Zhang F, Franzblau SG, Lewis WH, McNeil MR,
Drug targeting Mycobacterium tuberculosis cell wall synthesis: genetics of dTDP -rhamnose synthetic enzymes and development of a microtiter plate-based screen for inhibitors of conversion of dTDP -glucose to dTDP-rhamnose
Antimicrob Agents Chemother (2001) 45(5):1407-16
Ma Y, Pan F, McNeil M,
Formation of dTDP-Rhamnose Is Essential for Growth of Mycobacteria
J Bacteriol (2002) 184(12):3392-5
Valvano MA, Messner P, Kosma P,
Novel pathways for biosynthesis of nucleotide-activated glycero -manno-heptose precursors of bacterial glycoproteins and cell surface polysaccharides
Microbiology (2002) 148(Pt 7):1979-89
Belanger AE, Inamine JM,
Molecular Genetics of Mycobacteria; Twelfth chapter: Genetics of Cell Wall Biosynthesis
ASM Press (2000) 1-55581-191-4:191-202