Quick Search

Enter gene name or accession number


Search term: Rv3216

General annotation | Coordinates | Sequence | Structural information | Orthologs/Cross-references | Interacting Drugs/Compounds | Bibliography
Imagemap from R
To navigate upstream or downstream, click on the first or last gene

General annotation
Gene nameRv3216
Rv numberRv3216
TypeCDS (pseudogene)
FunctionAcetylation, substrate unknown
ProductGCN5-related N-acetyltransferase, pseudogene
CommentsRv3216, (MTCY07D11.10c), len: 160 aa. Acetyltransferase (2.3.1.-), contains GNAT domain (Gcn5-related N-acetyltransferase. See Vetting et al. 2005), probably pseudogene as appears frameshifted due to 1bp insertion at position 3593438. Frameshift present in all sequenced tubercle bacilli. Start changed since first submission, extended by 50aa. Similar to many acetyltransferases e.g. Q9AB32|CC0402 acetyltransferase (GNAT family) from Caulobacter crescentus (159 aa), FASTA scores: opt: 325, E(): 3.8e-17, (45.65% identity in 103 aa overlap); P79081|ATS1 putative acetyltransferase ATS1 from Schizosaccharomyces pombe (Fission yeast) (168 aa), FASTA scores: opt: 313, E(): 3.1e-16, (47.6% identity in 105 aa overlap).
Molecular mass (Da)17898.3
Isoelectric point6.5101
Gene length (bp)483
Protein length160
Location (kb)3593.37


Functional categoryintermediary metabolism and respiration


Mutationnon essential gene by Himar1-based transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website


Coordinates
TypeStartEndOrientation
CDS35933693593852+
RBS35935053593508+


Protein sequence in FASTA format
No protein sequence available for pseudogenes
Genomic sequence

Add extra bases upstream (5') and downstream (3')



Structural information
Protein Data BankNo structure available
PFAMO05850


Orthologs/Cross-references
CDC1551MT3312
Enzyme Classification2.3.1.-
Gene OntologyN-acetyltransferase activity
metabolic process
M. bovisMb3242
M. lepraeML0807
M. marinumMMAR_1341
M. smegmatisMSMEG_1924
UniProtO05850
Multiple Sequences Alignment: between orthologs


Interacting Drugs/Compounds
TDR TargetsRv3216


Expression Data
TBDBRv3216


Bibliography
Sassetti CM, Boyd DH, Rubin EJ,
Genes required for mycobacterial growth defined by high density mutagenesis.
Mol Microbiol (2003) 48(1):77-84
Cited for: Mutant
Vetting MW, S de Carvalho LP, Yu M, Hegde SS, Magnet S, Roderick SL, Blanchard JS,
Structure and functions of the GNAT superfamily of acetyltransferases
Arch Biochem Biophys (2005) 433(1):212
Cited for: Biochemistry
Griffin JE, Gawronski JD, Dejesus MA, Ioerger TR, Akerley BJ, Sassetti CM,
High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism.
PLoS Pathog (2011) 7(9):e1002251
Cited for: Mutant