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Search term: Rv2247

General annotation | Coordinates | Sequence | Structural information | Orthologs/Cross-references | Interacting Drugs/Compounds | Bibliography
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General annotation
Gene nameaccD6
Rv numberRv2247
FunctionInvolved in fatty acid biosynthesis (mycolic acids synthesis) [catalytic activity: ATP + propionyl-CoA + CO(2) + H(2)O = ADP + orthophosphate + methylmalonyl-CoA].
ProductAcetyl/propionyl-CoA carboxylase (beta subunit) AccD6
CommentsRv2247, (MTCY427.28), len: 473 aa. AccD6, Acetyl/Propionyl CoA Carboxylase, beta subunit (see citations below), highly similar to e.g. PCCB_RHOSO|Q06101 propionyl-CoA carboxylase beta chain, FASTA score: (75.1% identity in 437 aa overlap). Similar to many other Acetyl/Propionyl CoA Carboxylases from Mycobacterium tuberculosis. Belongs to the AccD / PccB family.
Molecular mass (Da)50136
Isoelectric point6.0864
Gene length (bp)1422
Protein length473
Location (kb)2520.74

Functional categorylipid metabolism

ProteomicsIdentified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). Translational start site supported by proteomics data (See Kelkar et al., 2011).
TranscriptomemRNA identified by DNA microarray analysis (gene induced by isoniazid (INH) or ethionamide treatment) (see Wilson et al., 1999). mRNA also identified by other microarray analysis and real-time RT-PCR; transcription up-repressed at low pH in vitro conditions, which may mimic an environmental signal encountered by phagocytosed bacteria (see Fisher et al., 2002). mRNA also identified by microarray analysis and up-regulated after 96h of starvation (see Betts et al., 2002).
Mutationessential gene by Himar1-based transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website


Protein sequence in FASTA format
>M. tuberculosis H37Rv|Rv2247|accD6
Blastp: Pre-computed results
TransMembrane prediction using Hidden Markov Models: TMHMM
Genomic sequence

Add extra bases upstream (5') and downstream (3')

Structural information
Protein Data BankNo structure available

Enzyme Classification6.4.1.3
Gene Ontologypropionyl-CoA carboxylase activity
ATP binding
M. bovisMb2271
M. lepraeML1657
M. marinumMMAR_3340
M. smegmatisMSMEG_4329
Multiple Sequences Alignment: between orthologs

Interacting Drugs/Compounds
TDR TargetsRv2247
Drug Resistance MutationsIsoniazid

Expression Data

Wilson M, DeRisi J, Kristensen HH, Imboden P, Rane S, Brown PO, Schoolnik GK,
Exploring drug-induced alterations in gene expression in Mycobacterium tuberculosis by microarray hybridization
Proc Natl Acad Sci U S A (1999) 96(22):12833-8
Cited for: Regulation
Betts JC, Lukey PT, Robb LC, McAdam RA, Duncan K,
Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling
Mol Microbiol (2002) 43(3):717-31
Cited for: Transcriptome
Fisher MA, Plikaytis BB, Shinnick TM,
Microarray analysis of the Mycobacterium tuberculosis transcriptional response to the acidic conditions found in phagosomes
J Bacteriol (2002) 184(14):4025-32
Cited for: Transcriptome/Regulation
Sassetti CM, Boyd DH, Rubin EJ,
Genes required for mycobacterial growth defined by high density mutagenesis.
Mol Microbiol (2003) 48(1):77-84
Cited for: Mutant
Kruh NA, Troudt J, Izzo A, Prenni J, Dobos KM,
Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo.
PLoS One (2010) 5(11):e13938
Cited for: Proteomics
Kelkar DS, Kumar D, Kumar P, Balakrishnan L, Muthusamy B, Yadav AK, Shrivastava P, Marimuthu A, Anand S, Sundaram H, Kingsbury R, Harsha HC, Nair B, Prasad TS, Chauhan DS, Katoch K, Katoch VM, Kumar P, Chaerkady R, Ramachandran S, Dash D, Pandey A,
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.
Mol Cell Proteomics (2011) 10(12):M111.011627
Cited for: Proteomics/Sequence
Griffin JE, Gawronski JD, Dejesus MA, Ioerger TR, Akerley BJ, Sassetti CM,
High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism.
PLoS Pathog (2011) 7(9):e1002251
Cited for: Mutant
Cole ST, Brosch R, Parkhill J, Garnier T, Churcher C, Harris D, Gordon SV, Eiglmeier K, Gas S, Barry CE 3rd, Tekaia F, Badcock K, Basham D, Brown D, Chillingworth T, Connor R, Davies R, Devlin K, Feltwell T, Gentles S, Hamlin N, Holroyd S, Hornsby T, Jagels K, Barrell BG, et al,
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence
Nature (1998) 393(6685):537-44
Cited for: Sequence/Secondary
Kremer L, Baulard AR, Besra GS,
Molecular Genetics of Mycobacteria; Eleventh chapter: Genetics of Mycolic Acid Biosynthesis
ASM Press (2000) 1-55581-191-4:173-190
Cited for: Review