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Search term: Rv1603

General annotation | Coordinates | Sequence | Structural information | Orthologs/Cross-references | Interacting Drugs/Compounds | Bibliography
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General annotation
Gene namehisA
Rv numberRv1603
FunctionHistidine biosynthesis pathway (fourth step) [catalytic activity: N-(5'-phospho-D-ribosylformimino)-5-amino-1-(5''-phosphoribosyl)-4-imidazolecarb oxamide = N-(5'-phospho-D-1'-ribulosylformimino)-5-amino-1-(5''-phosphoribosyl)-4- imidazolecarboxamide.]
ProductProbable phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase HisA
CommentsRv1603, (MTV046.01-MTCY336.01c), len: 245 aa. Probable hisA, phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase, similar to many e.g. HIS4_STRCO|P16250 phosphoribosylformimino-5-aminoimidaz from Streptomyces coelicolor (240 aa), FASTA scores: opt: 1081, E(): 0, (69.0% identity in 239 aa overlap).
Molecular mass (Da)25730.9
Isoelectric point4.4174
Gene length (bp)738
Protein length245
Location (kb)1803.29

Functional categoryintermediary metabolism and respiration

ProteomicsIdentified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011).
Mutationessential gene by Himar1-based transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website


Protein sequence in FASTA format
>M. tuberculosis H37Rv|Rv1603|hisA
Blastp: Pre-computed results
TransMembrane prediction using Hidden Markov Models: TMHMM
Genomic sequence

Add extra bases upstream (5') and downstream (3')

Structural information
Protein Data Bank2Y85 2Y88 2Y89

Enzyme Classification5.3.1.16
Gene Ontologyhistidine biosynthetic process
tryptophan biosynthetic process
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity
phosphoribosylanthranilate isomerase activity
M. bovisMb1629
M. lepraeML1261
M. marinumMMAR_2399
M. smegmatisMSMEG_3209
Multiple Sequences Alignment: between orthologs

Interacting Drugs/Compounds
TDR TargetsRv1603

Expression Data

Barona-Gomez F, Hodgson DA,
Occurrence of a putative ancient-like isomerase involved in histidine and tryptophan biosynthesis.
EMBO Rep (2003) 4(3):296-300
Cited for: Product/Function/Homolog/Secondary
Sassetti CM, Boyd DH, Rubin EJ,
Genes required for mycobacterial growth defined by high density mutagenesis.
Mol Microbiol (2003) 48(1):77-84
Cited for: Mutant
Malen H, Pathak S, Softeland T, de Souza GA, Wiker HG,
Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv.
BMC Microbiol (2010) 10:132
Cited for: Proteomics
de Souza GA, Leversen NA, Malen H, Wiker HG,
Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway.
J Proteomics (2011) 75(2):502-10
Cited for: Proteomics
Griffin JE, Gawronski JD, Dejesus MA, Ioerger TR, Akerley BJ, Sassetti CM,
High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism.
PLoS Pathog (2011) 7(9):e1002251
Cited for: Mutant