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Gene: Rv0012

General annotation | Coordinates | Sequence | Genome Browser | Structural information | Orthologs/Cross-references | Interacting Drugs/Compounds | Bibliography


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General annotation
Gene nameRv0012
Rv numberRv0012
TypeCDS
FunctionUNKNOWN
ProductPROBABLE CONSERVED MEMBRANE PROTEIN
CommentsRv0012, (MTCY10H4.12), len: 262 aa. Probable conserved membrane protein, similar to AL079308|SCH69_23|T36722 hypothetical protein from Streptomyces coelicolor (237 aa), FASTA scores: opt: 506, E(): 1.9e-25, (39.8% identity in 236 aa overlap). Some similarity to BLU0|1958_35A2 DIVIB (fragment) (188 aa), FASTA scores: opt: 204, E(): 8.9e-07, (35.6% identity in 90 aa overlap); and G1129091|DDS cell division and sporulation protein from Bacillus subtilis (231 aa), FASTA scores: opt: 180, E(): 3.8e-05, (30.7% identity in 101 aa overlap). Also similar to Rv1823|MTCY1A11_20 from Mycobacterium tuberculosis FASTA score: (30.1% identity in 246 aa overlap); and MTCY1A11_18 FASTA score: (25.5% identity in 235 aa overlap). Contains probable N-terminal signal sequence.
Molecular mass (Da)28337.4
Isoelectric point7.9985
Gene length (bp)789
Protein length262
Location (kb)14.089


Functional categorycell wall and cell processes


ProteomicsPredicted secreted protein - identified in culture filtrates of M. tuberculosis H37Rv; signal peptide predicted (See Malen et al., 2007). Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005).
Mutationnon essential gene by Himar1-based transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003) mutants available at TARGET website
Annotation Changesproteomics updated on 01-JAN-2009


Coordinates
TypeStartEndOrientation
CDS1408914877+


Protein sequence in FASTA format
>M. tuberculosis H37Rv | Rv0012
MRLTHPTPCPENGETMIDRRRSAWRFSVPLVCLLAGLLLAATHGVSGGTEIRRSDAPRLVDLVRRAQASVNRLATEREAL
TTRIDSVHGRSVDTALAAMQRRSAKLAGVAAMNPVHGPGLVVTLQDAQRDANGRFPRDASPDDLVVHQQDIEAVLNALWN
AGAEAIQMQDQRIIAMSIARCVGNTLLLNGRTYSPPYTIAAIGDAAAMQAALAAAPLVTLYKQYVVRFGLGYCEEVHPDL
QIVGYADPVRMHFAQPAGPLDY
Blastp: results
TransMembrane prediction using Hidden Markov Models: tmhmm
Microbe Genome Browser
Genomic sequence

Add extra bases upstream (5') and downstream (3')



Structural information
Protein Data BankNo structure available
PFAMProtein Family Domains


Orthologs/Cross-references
CDC1551MT0015
MbovisMb0012
MlepraeML0014
MsmegmatisMSMEG_0027
UniProtP71582


Interacting Drugs/Compounds
TDR TargetsRv0012


Bibliography
Sassetti CM, Boyd DH, Rubin EJ,
Genes required for mycobacterial growth defined by high density mutagenesis.
Mol Microbiol (2003) 48(1):77-84
Mawuenyega KG, Forst CV, Dobos KM, Belisle JT, Chen J, Bradbury EM, Bradbury AR, Chen X,
Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling.
Mol Biol Cell (2005) 16(1):396-404
Malen H, Berven FS, Fladmark KE, Wiker HG,
Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv.
Proteomics (2007) 7(10):1702-18